Abstracts (first author)
Effects of experimental mating system variation on transcriptome evolution and mating response in female Drosophila pseudoobscura
Interactions between the sexes are believed to be a potent source of selection on sex-specific evolution, but the way in which sexual interactions influence females at the molecular level is poorly understood. We examined the effects of 100 generations of elevated polyandry and enforced monandry on gene expression evolution in female Drosophila pseudoobscura. We assessed differences in gene expression between females from the two selection regimes and how the expression response to mating depended on female sexual selection history, indirect genetic effects (IGEs) due to the genotype of the male and female-by-male genotype interactions. Our data show large-scale gene expression divergence between the experimentally evolved females, with selection mainly targeting female-biased genes expressed in the ovaries. Most of the expression differences were un-affected by mating, however we also observed substantial differences in the expression response to mating between the experimental females. The indirect genetic effects of the male selection history on the female gene expression response were surprisingly small. Our results provide critical experimental evidence for a role of female-specific selection arising from polyandry in promoting rapid evolution of the female transcriptome.