Abstracts (first author)

Talk 

Shifting fitness landscapes in response to altered environments

Author(s): Bank C, Hietpas RT, Bolon DNA, Jensen JD

Summary:

One of the most controversial questions in evolutionary biology is the role of adaptation in molecular evolution. Following decades of debate between selectionists and neutralists, new high-throughput methods are beginning to illuminate the full distribution of fitness effects of new mutations. Here, we shed light on the adaptive potential in Saccharomyces cerevisiae by presenting systematic high-throughput fitness measurements for 578 possible point mutations in a region of Hsp90 in each of six environmental conditions. Under elevated salinity, we observe numerous beneficial mutations with growth advantages up to 7% relative to the wild type. We thus demonstrate that an essential protein can harbor adaptive potential upon an environmental challenge, and report a remarkable fit of the data to Fisher's geometric model - which well predicts the observed costs of adaptation associated with environmentally specific beneficial mutations. Furthermore, we utilize a Bayesian MCMC modeling framework to evaluate the statistical significance of the results - showing a remarkable accuracy of the experimental approach, e.g., allowing for direct inference of the non-neutrality of certain synonymous substitutions.


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Contacts

Chairman: Octávio S. Paulo
Tel: 00 351 217500614 direct
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email: mail@eseb2013.com

Address

XIV Congress of the European Society for Evolutionary Biology

Organization Team
Department of Animal Biology (DBA)
Faculty of Sciences of the University of Lisbon
P-1749-016 Lisbon
Portugal

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Computational Biology & Population Genomics Group 
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